Thermo Fisher Scientific Invitrogen Platinum SYBR Green qPCR SuperMix-UDG

Thermo Fisher Scientific Invitrogen Platinum SYBR Green qPCR SuperMix-UDG

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Product Details:

Storage Instructions Store at -20 C for long-term stability
Function Enables sensitive DNA or cDNA quantification via real-time PCR
Shelf Life 12 months (when stored properly)
Measurement Range Dynamic range typically: 68 orders of magnitude (depending on instrument and assay)
Accuracy High sensitivity and specificity for qPCR applications
Features Contains Platinum Taq DNA Polymerase, SYBR Green I dye for double-stranded DNA detection, UDG (uracil-DNA glycosylase) for prevention of carryover contamination, optimized buffer system, high sensitivity
Usage Type Laboratory/Research
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Thermo Fisher Scientific Invitrogen Platinum SYBR Green qPCR SuperMix-UDG Price And Quantity

Thermo Fisher Scientific Invitrogen Platinum SYBR Green qPCR SuperMix-UDG Product Specifications

Features Contains Platinum Taq DNA Polymerase, SYBR Green I dye for double-stranded DNA detection, UDG (uracil-DNA glycosylase) for prevention of carryover contamination, optimized buffer system, high sensitivity
Storage Instructions Store at -20 C for long-term stability
Accuracy High sensitivity and specificity for qPCR applications
Real-Time Operation Yes
Technology Real-Time PCR with SYBR Green I dye detection
Dimension (L*W*H) Not applicable (reagent bottle/tube format)
Function Enables sensitive DNA or cDNA quantification via real-time PCR
Use Quantitative PCR (qPCR) and quantitative reverse-transcription PCR (qRT-PCR) for DNA and cDNA target detection
Measurement Range Dynamic range typically: 68 orders of magnitude (depending on instrument and assay)
Power Source Not required (used as a reagent in compatible qPCR cyclers)
Portable Yes (reagent format)
Instruments Type Compatible with most real-time PCR instruments
Shelf Life 12 months (when stored properly)
Condition New
Wall Mounted No
Usage Type Laboratory/Research
Material Liquid reagent; primarily buffer and enzyme solution
Color Light green (due to SYBR Green dye)
Equipment Type qPCR SuperMix
Operating Type Ready-to-use master mix for real-time PCR

Template Compatibility Genomic DNA, cDNA, and synthetic DNA templates
Dye System SYBR Green I, intercalating dsDNA dye
Contamination Prevention Uracil-DNA glycosylase (UDG) included to prevent carryover contamination
Reaction Volume Flexible, typically 1050 L
Master Mix Format 2X concentrated, ready-to-use supermix
Enzyme Platinum Taq DNA Polymerase, uracil-DNA glycosylase (UDG) included
Cat. Number 11733038 (example, check manufacturer's catalog for specifics)
Application Gene expression analysis, genetic variation analysis, DNA quantification, molecular diagnostics (research use only)
PCR Product Visualization Real-time fluorescence detection
Shipping Condition Ships on dry ice

Product Description

Brand	Thermo Fisher Scientific
Product Type	qPCR Kit
Pack Size	500 Reactions
CAT No	11733046
Application	Molecular Biology

Platinum SYBR Green qPCR SuperMix-UDG provides a sensitive and convenient system for real-time PCR detection with SYBR Green I dye. The SuperMix format combines amplification and detection reagents into a ready-to-go mix to make set-up easy and ensure reproducible results.

Designed for Precision and Convenience

This master mix is pre-formulated to simplify real-time PCR set-up, ensuring reproducibility and minimizing variation. The light green appearance indicates the presence of SYBR Green I for double-stranded DNA detection, while UDG minimizes cross-contamination, making it suitable for meticulous laboratory workflows.

Versatile Template Compatibility

Whether your research involves genomic DNA, cDNA, or synthetic DNA, the Platinum SYBR Green qPCR SuperMix-UDG ensures reliable performance. Its flexible reaction volume (10-50 L) enables adaptation to diverse experimental needs while maintaining consistency across a broad dynamic range.

Robust Results for Multiple Applications

From gene expression analysis and DNA quantification to genetic variation detection, this master mix supports a spectrum of real-time PCR applications. Its optimized buffer system guarantees high specificity and sensitivity, producing accurate results even at low target concentrations.

FAQ's of Thermo Fisher Scientific Invitrogen Platinum SYBR Green qPCR SuperMix-UDG:

Q: How does the Invitrogen Platinum SYBR Green qPCR SuperMix-UDG prevent carryover contamination?

A: This supermix includes uracil-DNA glycosylase (UDG), which selectively removes uracil-containing DNA introduced from previous PCR amplifications, thereby greatly reducing the risk of contamination due to amplicon carryover.

Q: What templates can be used with this qPCR SuperMix?

A: The supermix is compatible with a broad range of DNA templates, including genomic DNA, cDNA, and synthetic DNA, making it versatile for various genetic and molecular applications.

Q: When should I use the Platinum SYBR Green qPCR SuperMix-UDG?

A: Use this master mix when you require sensitive, specific detection and quantification of DNA or cDNA targets in real-time, such as during gene expression analysis, DNA quantification, or genetic variation studies.

Q: Where should the reagent be stored for optimal stability?

A: For long-term preservation of stability and performance, store the reagent at -20C. The product ships on dry ice to ensure appropriate conditions are maintained during delivery.

Q: What is the recommended process for setting up a reaction using this supermix?

A: Simply mix the 2X supermix with your desired DNA or cDNA template and primers according to your target protocol. The mix is ready-to-use, reducing setup time and risk of error.

Q: What are the benefits of using SYBR Green I for DNA detection in this master mix?

A: SYBR Green I is an intercalating dye that fluoresces upon binding to double-stranded DNA, enabling direct visualization and quantification of PCR product accumulation in real-time, improving both sensitivity and accuracy.

Q: Is the Platinum SYBR Green qPCR SuperMix-UDG compatible with most real-time PCR instruments?

A: Yes, this master mix is formulated to work efficiently with a wide range of real-time PCR cyclers commonly found in research laboratories.

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