Product Description
Brand Thermo Fisher Scientific
Product Type Electroporation Kit
Pack Size 96 x 2 Reactions
CAT No N10096
Application Blotting Kit
The Neon NxT Electroporation System 100-I14L Kit with 1-Channel Tubes is designed specifically for use with the Neon NxT Electroporation System (Cat. Nos. NEON1S, NEON1SK, NEON18S, NEON18SK). Use this kit for transfection volumes of 100 I14L, containing 5 x 105a2 x 106 adherent cells or 1 x 106a5 x 106 suspension cells. Cells that have been transfected using the included 100-I14L Neon NxT tips are then ready to be washed and plated into a multi-well culture dish.
Advanced Electroporation Technology for Precision TransfectionThe Neon NxT Electroporation System incorporates proprietary Neon Transfection Technology, offering unparalleled control and consistency in cell transfection outcomes. With real-time operation, customizable programs, and single-shot sample loading, researchers can achieve high transfection efficiency across a wide range of mammalian cells. The gold-plated electrodes and automated safety features further enhance operational reliability and sample integrity.
Customizable Protocols and Seamless User ExperienceThis system provides flexibility with user-defined and stored protocols, enabling optimized transfection conditions for diverse applications. The intuitive touchscreen LCD simplifies operation, protocol programming, and traceable electroporation, while USB connectivity supports easy protocol transfer and software updates. Designed to meet varied research needs, it accommodates sample volumes up to 100 L with one-channel tips.
Reliable Safety and Robust PerformanceSafety is prioritized through features such as auto-lid lock, overcurrent protection, and voltage detection. Operating quietly and efficiently within a wide temperature and humidity range, the system is suitable for demanding laboratory conditions. Its accuracy, reliable performance, and low noise operation make it a valuable asset for laboratories focused on precision and reproducibility.
FAQ's of Thermo Fisher Scientific Invitrogen Neon NxT Electroporation System 100-microliter Kit with 1-Channel Tubes:
Q: How does the Invitrogen Neon NxT system deliver nucleic acids into cells?
A: The Neon NxT Electroporation System transfers nucleic acids into mammalian cells by generating controlled electrical pulses. This process creates temporary pores in the cell membrane, enabling the introduction of DNA, RNA, or proteins. The system features up to three consecutive pulses per formulation, ensuring high transfection efficiency.
Q: What sample types and volumes are compatible with the Neon NxT Electroporation System?
A: This system is compatible with mammalian cells, including stem cells, primary cells, and common cell lines. It utilizes 100 L 1-channel tubes, allowing processing of single samples per channel during each run.
Q: When should I use customizable electroporation protocols?
A: Customizable and saved protocols are ideal when working with different cell types or optimizing conditions for new experimental setups. The system allows you to program, store, and retrieve protocols, enabling reproducible results for varied research needs.
Q: Where is the Invitrogen Neon NxT system best utilized?
A: The Neon NxT system is ideal for laboratory and research settings, particularly in applications requiring efficient delivery of nucleic acids into sensitive or hard-to-transfect mammalian cells.
Q: What is the process for loading and operating the system?
A: Single-shot loading allows straightforward sample introduction into the 1-channel 100 L tip. Operation is automatic, guided by the touchscreen LCD, with safety checks like auto-lid locking and voltage detection for secure handling. User-defined programs can be selected and run for tailored electroporation.
Q: How does the system benefit research and lab workflows?
A: With high transfection efficiency, programmable protocols, and traceable electroporation, the Neon NxT system supports reliable and reproducible results. Its compact, portable design and low-noise operation streamline workflows in busy labs or collaborative research environments.
Q: What safety and connectivity features does the system provide?
A: Equipped with an auto-lid lock, overcurrent protection, and voltage detection, the system ensures operator and sample safety. USB connectivity enables easy software updates and protocol transfer, enhancing usability and convenience.